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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which results in chromosome instability, and so might add to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a vital job in advertising and marketing mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are explained. Two additional splice variants had been reported but in depth nucleotide sequence facts is not however offered.
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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide usually contains the epitope identified through the antibody.
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This guide outlines further more possible optimization of mobile and immunoassay parameters to be certain the very best benefits are...
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This guide outlines further possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
Antibodies sure to the blocking peptide no longer bind into the epitope about the target protein. By evaluating the staining from your blocked antibody vs . the antibody on your own, you can see which staining is particular.
Concentrate on Details Cyclin E belongs to the hugely conserved cyclin family members, whose customers are characterized by a remarkable periodicity in protein abundance in the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation designs which lead into the temporal coordination of each mitotic function. Cyclin E kinds a posh with and capabilities as a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is degraded as cells development through S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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